Deadly New Brain Virus – Dr John Campbell January 12, 2024 Unipro2013main Health, Healthcare, News, World News </p> <h2>New brain virus</h2> <p> 100% lethal. Time to ban this reaearch.<br /> Lethal Infection of Human ACE2-Transgenic Mice Caused by SARS-CoV-2- related Pangolin Coronavirus GX_P2V(short_3UTR)<br /> 4th January 2024</p> <p>https://www.biorxiv.org/content/10.1101/2024.01.03.574008v1.full</p> <p>https://www.biorxiv.org/content/10.1101/2024.01.03.574008v1.full.pdf</p> <p>SARS-CoV-2-related pangolin coronavirus GX_P2V can cause 100% mortality in human ACE2-transgenic mice,</p> <p>potentially attributable to late-stage brain infection.</p> <p>This underscores a spillover risk of GX_P2V into humans,</p> <p>and provides a unique model for understanding the pathogenic mechanisms of SARS-CoV-2-related viruses.</p> <p>Dear Editor,</p> <p>Two SARS-CoV-2-related pangolin coronaviruses, GD/2019 and GX/2017,</p> <p>were identified prior to the COVID-19 outbreak.<br /> The respective isolates, were cultured in 2020 and 2017</p> <p>the early passaged GX_P2V isolate was actually a cell culture-adapted mutant.</p> <p>We assessed its pathogenicity in hACE2 mice.</p> <p>We found that the GX_P2V clone can infect hACE2 mice,</p> <p>with high viral loads detected in both lung and brain tissues.</p> <p>This infection resulted in 100% mortality in the hACE2 mice.</p> <p>We surmise that the cause of death may be linked to the occurrence of late brain infection.</p> <p>All the mice that were infected with the live virus succumbed to the infection within 7-8 days post-inoculation,</p> <p>rendering a mortality rate of 100%</p> <p>5 days post-infection</p> <p>decrease in body weight</p> <p>7 days post-infection</p> <p>piloerection, hunched posture, and sluggish movements, and their eyes turned white.</p> <p>8 hACE2 mice were infected</p> <p>8 mice inoculated with inactivated virus</p> <p>8 mice mock-infected</p> <p>Dissections on days 3 and 6</p> <p>Quantitative analysis of viral RNA and titer.</p> <p>Significant amounts of viral RNA in the brain, lung, turbinate, eye, and trachea</p> <p>no or a low amount of viral RNA was detected in heart, liver, spleen, kidneys, tongue, stomach, and intestines.</p> <p>Lung samples</p> <p>High viral RNA loads on days 3 and 6 post-infection</p> <p>Viral antigens also detected</p> <p>Viral load in the lungs significantly decreased by day 6</p> <p>Lungs showed minimal inflammation</p> <p>Brain samples</p> <p>Day 3 post-infection, viral RNA was detected in all infected mice,</p> <p>shrunken neurons visible in cerebral cortex</p> <p>Viral antigens also detected</p> <p>Day 6 post-infection, exceptionally high viral RNA loads (∼ 8.5 Log10[copies/mg]) in the brain samples from all infected mice,</p> <p>focal lymphocytic infiltration around the blood vessels.</p> <p>Severe brain infection during the later stages of infection may be the key cause of death in these mice.</p> <p>To the best of our knowledge, this is the first report showing that a SARS-CoV-2-related pangolin coronavirus can cause 100% mortality in hACE2 mice,</p> <p>suggesting a risk for GX_P2V to spill over into humans.</p> <p>Propensity of coronaviruses to undergo adaptive mutation during passage culture.</p> <p>……. requires further investigation.</p> <p>It is possible that GX_P2V C7 has undergone a virulence-enhancing mutation.</p> <p>Justin Kinney, Simons Center for Quantitative Biology, Cold Spring Harbor Laboratory, U.S.</p> <p>“The research is still very dangerous, though,”</p> <p>“I am especially concerned that the paper does not say what biosafety level the work was performed at.</p> <p>Coronavirus research in China is often done at a biosafety level (BSL-2) that is inadequate for working with potential pandemic pathogens that might be transmitted by air.</p> <p>“Indeed, coronavirus research done at BSL-2 may have caused the COVID-19 pandemic.</p> <p>And by showing that the coronavirus has a surprisingly high pathogenicity, the work underscores the need for extreme caution when working with novel coronaviruses.”</p> <p>https://www.theepochtimes.com/health/chinese-scientists-reveal-experiments-with-virus-100-percent-fatal-to-mice-5560854?utm_source=healthnoe&src_src=healthnoe&utm_campaign=health-2024-01-11&src_cmp=health-2024-01-11&utm_medium=email&est=WbDdGa4Q6Iq698FpcqUs8Zg1gnKWQsOyvg6pqjrDjV5egMs753Fg8CTOlo2Dgu4ObrfZxQ%3D%3D</p> <p>Lihua Song</p> <p>did not respond to a request for comment on how the scientists ensured the experiments they performed were safe.</p> <p>Yigang Tong</p> <p>Trained in a Chinese military program and worked in military-run labs.</p> <p>He also co-authored a paper in 2023 with Zheng-Li Shi, who helps run the Wuhan Institute of Virology.</p> <p>https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9972989/</p> <p>Justin Goodman, senior vice president, White Coat Waste Project, a U.S. nonprofit</p> <p>“dangerous and deadly tests on mice.”</p> <p>“This is why shipping US tax dollars to foreign adversaries’ unaccountable animal labs is a recipe for disaster and we’re working with lawmakers to stop it,”<br /> Credit to : Dr. John Campbell </p> <p> Please support our Sponsors here :
